Based on a popular course at Cold Spring Harbor Laboratory this new manual assembles cuttingedge protocols helpful hints and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using stateoftheart instrumentation. Detailed protocols involving protein microarrays liquid chromatography highthroughput cloning of expression constructs IMAC mass spectrometry MALDITOF and MudPIT are provided along with wellillustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. Proteomics A Cold Spring Harbor Laboratory Course Manual can be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments.